RESUMO
The present study was designed to investigate the antihyperglycemic effect of Persea duthieion blood glucose concentration and body weight in alloxan induced diabetic hyperglycemic rabbits. The results illustrated significant antihyperglycemic activity of crude extract with 17.44% and 28.02% amelioration at 25 and 50mg/kg p.o. respectively after 24th day of drug treatment; equally supported by body weight recovery. Upon fractionation, most dominant antihyperglycemic effect was displayed by aqueous fraction with 22.12% and 34.43% effect followed by ethyl acetate fraction with 24.32% and 32.05% effect at 25 and 50mg/kg p.o. respectively after 24th day of drug treatment. The effect on blood glucose was also reflected on body weight of animals. In conclusion, our study documented marked antihyperglycemic activity of extract/fractions of P. duthiei.
Assuntos
Aloxano , Glicemia/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Diabetes Mellitus Experimental/tratamento farmacológico , Hipoglicemiantes/farmacologia , Persea/química , Extratos Vegetais/farmacologia , Acetatos/química , Animais , Biomarcadores/sangue , Glicemia/metabolismo , Fracionamento Químico , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/induzido quimicamente , Relação Dose-Resposta a Droga , Hipoglicemiantes/isolamento & purificação , Fitoterapia , Extratos Vegetais/isolamento & purificação , Plantas Medicinais , Coelhos , Solventes/química , Fatores de Tempo , Água/químicaRESUMO
Eruca sativa Miller is a worldwide common alimentary plant (rocket leaves). The aim of this study was to correlate the potential in vitro scavenging activity of the E. sativa hydroalcoholic extract (HAE) with its in vivo hypoglycaemic effect. In DDPH free radical (DFR) and ferric-reducing antioxidant power assays, HAE in a concentration dependent manner (25-100 µg/mL) displayed a strong scavenging activity with maximum effect of 88% and 75% at 100 µg/mL, respectively. Daily administration of HAE (50 mg/kg; p.o.) in the in vivo model of alloxan-induced diabetic rabbits for 28 days showed significant reduction in glycaemia, also supported by recovery of body weight. In conclusion, our results give preliminary information on the potential use of this plant as a nutraceutical, useful to control and/or prevent a hyperglycaemic status.
Assuntos
Brassicaceae/química , Diabetes Mellitus Experimental/tratamento farmacológico , Sequestradores de Radicais Livres/química , Hipoglicemiantes/química , Extratos Vegetais/química , Aloxano , Animais , Glicemia/análise , Peso Corporal , Diabetes Mellitus Experimental/induzido quimicamente , Sequestradores de Radicais Livres/isolamento & purificação , Hipoglicemiantes/isolamento & purificação , Folhas de Planta/química , CoelhosRESUMO
Caralluma tuberculata is a cooked food item in Pakistan especially for diabetics. The current study was designed to explore the antidiabetic potential of extract/fractions of Caralluma tuberculata in alloxan-induced diabetic rabbits and its effect on body weight. The crude extract of the plant provoked 24% and 44% antidiabetic action at 25 and 50 mg/kg OP, respectively, after the 24th day of treatment, which was strongly supported by a positive effect on the body weight of animals. On fractionation, pretreatment of the ethyl acetate fraction demonstrated most dominant (25.17% and 34.83%) antidiabetic activity followed by n-hexane (19.33% and 32.76%) and aqueous fractions (16.44% and 22.36%) at 25 and 50 mg/kg OP, respectively, after the 24th day of treatment. The corresponding effect on blood glucose was also observed on body weight of diabetic rabbits. In sum, extract/fractions of the plant showed marked antidiabetic action and thus a provided scientific foundation to the use of the plant as an antidiabetic.
